Infrared Identification in Paper Chromatography

The identification of barbiturates by paper chromatography.

B ECAUSE barbiturates are relatively easily available and much used drugs, specimens from suspected cases of poisoning are frequently submitted to the laboratory for routine chemical examination. Since their urinary excretion begins early (because they are quickly absorbed), they can often be identified 1-2 hours after administration. The specimen of choice for examination is therefore the urin...

Identification of Barbiturates in Blood by Paper Chromatography.

S TATISTICS conmpiled by time l’oison Control Center, New York City Department of Heaitlm, indicate fimat barbiturate imitoxication in adults ranks first as a cause of poisonilmg (1). The need for a rapid and sensitive method for detecting ammd ideiitifyiimg barbiturates in body fluids for diagnostic purpose is obvious. Even though timere Imave been mamiy analytic methods described in recelmt y...

Study of phenylketonuria by paper chromatography

Paper Chromatography

In this laboratory exercise we will separate and identify the cations in an aqueous mixture that possibly contains Fe 3+ , Ni 2+ or Cu 2+ salts. The separation will be achieved using Paper Chromatography. Identification of the cations will be affected by simultaneously running separate solutions, each containing a single salt of these ions, through the chromatographic system and comparing the r...

Identification of corticosteroids of beef adrenal extract by paper chromatography.

Chemical studies initiated in the laboratories of Reichstein, Kendall, and Wintersteiner (1) resulted in the isolation of twenty-eight crystalline steroids from adrenal gland extracts. Twelve of the compounds possessed an a-ketol side chain and, of these, six proved to be biologically active. The finding of 11-desoxycorticosterone, one of these active steroids, by Reichstein and von Euw (2) has...